Terpenoids are the most abundant compounds of plant secondary metabolites and have been interesting due to their pharmacological utility. įunding: The author(s) received no specific funding for this work.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The data that support the findings of this study are openly available in PRJNA464236, PRJNA464241 at and. Received: JAccepted: JanuPublished: July 7, 2023Ĭopyright: © 2023 Shams et al. PLoS ONE 18(7):Įditor: Mounir Tilaoui, Waterford Institute of Technology, IRELAND This study is the first report on de novo assembly and transcriptome data analysis in Satureja which could be useful for an understanding of the main constituents of Satureja essential oil and future research in this genus.Ĭitation: Shams S, Ismaili A, Firouzabadi FN, Mumivand H, Sorkheh K (2023) Comparative transcriptome analysis to identify putative genes involved in carvacrol biosynthesis pathway in two species of Satureja, endemic medicinal herbs of Iran. We confirmed the altered expression levels of DEGs that encode carvacrol biosynthetic enzymes using quantitative real-time PCR (qRT-PCR). In addition, we identified 19 differentially expressed transcription factors (such as MYC4, bHLH, and ARF18) that may control terpenoid biosynthesis. rechingeri transcripts involved in the terpenoid biosynthetic pathway were evaluated. 29 differentially expressed genes (DEGs) involved in terpenoid biosynthesis were identified, and these DEGs were significantly enriched in monoterpenoid biosynthesis, diterpenoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis, carotenoid biosynthesis and ubiquinone and other terpenoid-quinone biosynthesis pathways. 210 and 186 transcripts related to terpenoid backbone biosynthesis were identified for S. Cross-species differential expression analysis was conducted between two species of Satureja.

To identify the putative genes involved in carvacrol and other monoterpene biosynthesis pathway, we generated a reference transcriptome in two endemic Satureja species of Iran, containing different yields ( Satureja khuzistanica and Satureja rechingeri). However, limited information is available regarding the molecular mechanisms underlying carvacrol biosynthesis and its regulation for this wonderful medicinal herb. Satureja is rich in phenolic monoterpenoids, mainly carvacrol, that is of interest due to diverse biological activities including antifungal and antibacterial.

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